2RFV
High resolution structure of L-methionine gamma-lyase from Citrobacter freundii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-04-01 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.843 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 56.450, 122.800, 127.920 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.355 |
Rwork | 0.151 |
R-free | 0.17720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1y4i |
RMSD bond length | 0.008 |
RMSD bond angle | 1.021 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.400 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.034 | 0.340 |
Number of reflections | 95252 | |
<I/σ(I)> | 20.15 | 3.54 |
Completeness [%] | 95.6 | 84.5 |
Redundancy | 3.8 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 303 | 35% MMEPEG 2000, 50MM TRIS-HCL,0.2MM PLP, 0.25% DTT, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 303K |