2RFT
Crystal structure of influenza B virus hemagglutinin in complex with LSTa receptor analog
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 98.328, 98.328, 135.988 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 2.800 |
R-factor | 0.28175 |
Rwork | 0.280 |
R-free | 0.29870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Unliganded influenza B virus hemagglutinin |
RMSD bond length | 0.008 |
RMSD bond angle | 1.458 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 13.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.047 | 0.291 |
Number of reflections | 19145 | |
<I/σ(I)> | 16.6 | 2.1 |
Completeness [%] | 89.9 | 87.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 100 mM Pipes, 2.5 M ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |