2RFO

Crystral Structure of the nucleoporin Nic96

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-03
Detector	MAR CCD 165 mm
Wavelength(s)	0.9792
Spacegroup name	P 1
Unit cell lengths	62.000, 100.000, 104.000
Unit cell angles	62.00, 82.00, 86.00

Refinement procedure

Resolution	19.780 - 2.600
R-factor	0.23
Rwork	0.227
R-free	0.28500
Structure solution method	SAD
RMSD bond length	0.018
RMSD bond angle	1.694
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.670
High resolution limit [Å]	2.600	2.600
Number of reflections	64618
<I/σ(I)>	25.64	3.89
Completeness [%]	97.3	97.1
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	3-10% PEG3350, 0.1M BisTris pH6.5, 0.05M lithium sulfate, 3% 1,6-hexandiole, 0.01mM DTE, VAPOR DIFFUSION, HANGING DROP, temperature 293K