2RFM
Structure of a Thermophilic Ankyrin Repeat Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 180 |
Detector technology | CCD |
Collection date | 2006-07-12 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9238 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 101.002, 101.002, 174.483 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.650 |
R-factor | 0.157 |
Rwork | 0.156 |
R-free | 0.17800 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.373 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELX |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.472 | 61.660 | 1.740 |
High resolution limit [Å] | 1.650 | 5.220 | 1.650 |
Rmerge | 0.055 | 0.027 | 0.323 |
Total number of observations | 22182 | 37424 | |
Number of reflections | 58965 | ||
<I/σ(I)> | 7.2 | 19.9 | 2.4 |
Completeness [%] | 93.0 | 99.5 | 66.9 |
Redundancy | 9.9 | 9.8 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 286 | 20% Glycerol, 2M Ammoniumsulfate, 1% 1,3-butanediole, 20mM HEPES, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 286K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 286 | 20% Glycerol, 2M Ammoniumsulfate, 1% 1,3-butanediole, 20mM HEPES, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 286K |