2RFK
Substrate RNA Positioning in the Archaeal H/ACA Ribonucleoprotein Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 96.561, 96.561, 240.978 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.520 - 2.870 |
| R-factor | 0.25031 |
| Rwork | 0.248 |
| R-free | 0.30042 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ey4 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.725 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.520 | 2.945 |
| High resolution limit [Å] | 2.870 | 2.870 |
| Number of reflections | 20534 | |
| <I/σ(I)> | 41.9 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 15.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 303 | 50 mM MES, 100 mM NH4COOCH3, 5 mM MgSO4, 1.0 M NaCl, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MES | ||
| 2 | 1 | 1 | NH4COOCH3 | ||
| 3 | 1 | 1 | MgSO4 | ||
| 4 | 1 | 1 | NaCl | ||
| 5 | 1 | 2 | MES | ||
| 6 | 1 | 2 | NH4COOCH3 | ||
| 7 | 1 | 2 | MgSO4 | ||
| 8 | 1 | 2 | NaCl |
