2RFA
Crystal structure of the mouse TRPV6 ankyrin repeat domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.762, 63.045, 116.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.990 - 1.700 |
| R-factor | 0.17 |
| Rwork | 0.168 |
| R-free | 0.20700 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.372 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 1.742 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 25172 | |
| <I/σ(I)> | 18 | 3.4 |
| Completeness [%] | 99.1 | 95.34 |
| Redundancy | 7.5 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M NaHEPES, 0.1M K/Na Tartrate, 5% glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
