2REU
Crystal Structure of the C-terminal of Sau3AI fragment
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9789, 0.97945, 0.9 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 76.789, 122.009, 34.736 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.350 - 1.900 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.23600 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.031 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.13) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
| Rmerge | 0.071 | 0.047 | 0.433 |
| Number of reflections | 25678 | ||
| <I/σ(I)> | 10 | ||
| Completeness [%] | 96.4 | 99.8 | 77.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 8% iso-propanol, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
