2REI
Kinase domain of human ephrin type-A receptor 7 (Epha7)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-19 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 65 |
| Unit cell lengths | 51.880, 51.880, 215.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.950 - 1.600 |
| R-factor | 0.1637 |
| Rwork | 0.163 |
| R-free | 0.18678 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gsf |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.208 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 43283 | |
| <I/σ(I)> | 41.8 | 3.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.7 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | Well solution: 20% PEG 3350, 0.2 M NH4 formate, 0.1 M HEPES pH 7.0. Protein solution: 12 mg/mL. Drops: 1:1 ratio of well:protein solution. Crystals cryoprotected by transfer to drop containing mother liquor to which MPD was added to 25%, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
