2REE

Crystal structure of the loading GNATL domain of CurA from Lyngbya majuscula

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-06
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97939, 0.97934, 0.97945, 0.96112
Spacegroup name	P 31 2 1
Unit cell lengths	91.463, 91.463, 138.877
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.400 - 1.950
R-factor	0.174
Rwork	0.172
R-free	0.21100
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.382
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.10)
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.400	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.069	0.555
Number of reflections	49722
<I/σ(I)>	13.7	4.97
Completeness [%]	99.6	99.2
Redundancy	11.2	11.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	Mother liquor: 100 mM Bis-Tris pH 6.8, 100 mM NaCl, 1.5 M ammonium sulfate, 20% glycerol. Protein buffer: 20 mM Tris pH 7.9, 500 mM NaCl, 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K