2REB
THE STRUCTURE OF THE E. COLI RECA PROTEIN MONOMER AND POLYMER
Experimental procedure
| Spacegroup name | P 61 |
| Unit cell lengths | 102.400, 102.400, 82.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | ? - 2.300 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.600 |
| Phasing software | X-PLOR (2.1) |
| Refinement software | X-PLOR (2.1) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.300 * |
| Rmerge | 0.055 * |
| Completeness [%] | 92.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 5.4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 20 (mg/ml) | |
| 2 | 1 | 1 | citrate-phosphate | 0.1 (M) | |
| 3 | 1 | 1 | 0.1 (M) | ||
| 4 | 1 | 1 | glycerol | 10 (%) | |
| 5 | 1 | 1 | dithiothreitol | 0.1 (mM) | |
| 6 | 1 | 1 | 0.02 (%) | ||
| 7 | 1 | 1 | PEG8000 | 0.5 (%) | or 1 % |
| 8 | 1 | 2 | PEG8000 | 2 (%) | or 3 % |
