2RDU
Crystal Structure of Human Glycolate Oxidase in Complex with Glyoxylate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-06-13 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 4 |
| Unit cell lengths | 97.386, 97.386, 80.468 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.800 - 1.650 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gox |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.350 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK (9.2L) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.800 | 30.800 | 1.710 |
| High resolution limit [Å] | 1.650 | 3.550 | 1.650 |
| Rmerge | 0.073 | 0.029 | 0.412 |
| Total number of observations | 22707 | 12281 | |
| Number of reflections | 44625 | ||
| <I/σ(I)> | 11.6 | 35.5 | 2.7 |
| Completeness [%] | 98.8 | 99.9 | 88.8 |
| Redundancy | 4.57 | 4.85 | 3.08 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | HEPES,PEG 600,glyoxylate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
