2RDP
The structure of a MarR family protein from Bacillus stearothermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-01 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97906 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 73.643, 73.643, 90.432 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.100 - 2.300 |
| R-factor | 0.213 |
| Rwork | 0.211 |
| R-free | 0.25300 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.583 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.084 | 0.587 |
| Number of reflections | 11316 | |
| <I/σ(I)> | 9.2 | 2.3 |
| Completeness [%] | 97.3 | 89.8 |
| Redundancy | 6.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 291 | 0.056M NaH2PO4, 1.344M K2HPO4, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
