2RDI
Snapshots of a Y-family DNA polymerase in replication: Dpo4 in apo and binary/ternary complex forms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-05-04 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.927, 51.069, 86.026 |
| Unit cell angles | 90.00, 98.08, 90.00 |
Refinement procedure
| Resolution | 20.350 - 1.920 |
| R-factor | 0.207 |
| Rwork | 0.205 |
| R-free | 0.24700 |
| Starting model (for MR) | 1jx4 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.427 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.950 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.043 | 0.507 |
| Number of reflections | 28887 | |
| <I/σ(I)> | 16.8 | |
| Completeness [%] | 93.8 | 90.6 |
| Redundancy | 3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 294 | 20% PEG3350, 0.2M lithium nitrate, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
