2RDG
Crystal structure of Staphylococcal Superantigen-Like protein 11 in complex with Sialyl Lewis X
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 97.031, 57.945, 43.462 |
| Unit cell angles | 90.00, 101.83, 90.00 |
Refinement procedure
| Resolution | 22.450 - 1.600 |
| R-factor | 0.17576 |
| Rwork | 0.174 |
| R-free | 0.20656 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m4v |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.210 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.700 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.054 | 0.114 |
| Number of reflections | 28611 | |
| <I/σ(I)> | 10.1 | |
| Completeness [%] | 91.5 | 80.1 |
| Redundancy | 7.2 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 20% PEG3350, 0.2M potassium citrate, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
