2RD2
Glutaminyl-tRNA synthetase mutant C229R with bound analog 5'-O-[N-(L-GLUTAMINYL)-SULFAMOYL]ADENOSINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-02 |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 237.659, 93.337, 114.826 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 119.500 - 2.600 |
| Rwork | 0.209 |
| R-free | 0.26300 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 119.523 | 119.520 | 2.360 |
| High resolution limit [Å] | 2.264 | 10.290 | 2.330 |
| Rmerge | 0.084 | 0.031 | 0.582 |
| Total number of observations | 1291 | 1904 | |
| Number of reflections | 39063 | ||
| <I/σ(I)> | 7.3 | 21.6 | 1.2 |
| Completeness [%] | 71.2 | 69.8 | 47.5 |
| Redundancy | 2.3 | 2.7 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | Ammonium Sulfate, pH 7.5, VAPOR DIFFUSION, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Ammonium Sulfate | ||
| 2 | 1 | 1 | H2O | ||
| 3 | 1 | 2 | Ammonium Sulfate | ||
| 4 | 1 | 2 | H2O |
