2RD1

X-Ray structure of the protein Q7CQI7. Northeast Structural Genomics Consortium target StR87A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-09-11
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97900
Spacegroup name	P 41 21 2
Unit cell lengths	57.696, 57.696, 100.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.990 - 2.300
R-factor	0.207
Rwork	0.207
R-free	0.26400
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SnB
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.085	0.448
Number of reflections	14487
<I/σ(I)>	25.6	7.1
Completeness [%]	100.0	100
Redundancy	34.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.1	293	18% PEG 4000, 0.1M Ammonium chloride, pH 9.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K