2RCY
Crystal structure of Plasmodium falciparum pyrroline carboxylate reductase (MAL13P1.284) with NADP bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97937 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 160.207, 113.741, 82.651 |
| Unit cell angles | 90.00, 105.71, 90.00 |
Refinement procedure
| Resolution | 46.270 - 2.300 |
| R-factor | 0.247 |
| Rwork | 0.245 |
| R-free | 0.28900 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.941 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.270 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.142 | 0.496 |
| Number of reflections | 57815 | |
| <I/σ(I)> | 7.3 | 1.1 |
| Completeness [%] | 89.7 | 53.6 |
| Redundancy | 2.1 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 30% PEG MME550, 50 mM MgCl2, 0.1 M Hepes pH 7.5, 5 mM NADP, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
