2RCU
Crystal structure of rat carnitine palmitoyltransferase 2 in complex with r-3-(hexadecanoylamino)-4-(trimethylazaniumyl)butanoate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 95.273, 97.750, 312.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.780 |
| R-factor | 0.17978 |
| Rwork | 0.177 |
| R-free | 0.22331 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2deb |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.278 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.890 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.064 | 0.146 |
| Number of reflections | 126696 | |
| <I/σ(I)> | 22.26 | 12.71 |
| Completeness [%] | 91.0 | 95.6 |
| Redundancy | 7.2 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 0.15 M DL-malic acid pH 7.0, 20% (w/v) PEG 3350 (Index 91, Hampton Research), VAPOR DIFFUSION, HANGING DROP, temperature 294K |
