2RCQ
Crystal structure of human apo Cellular Retinol Binding Protein II (CRBP-II)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-05-12 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.518, 49.834, 74.398 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.200 |
R-factor | 0.197 |
Rwork | 0.196 |
R-free | 0.21700 |
Starting model (for MR) | 1opa |
RMSD bond length | 0.007 |
RMSD bond angle | 1.044 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.000 | 1.230 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.047 | 0.187 |
Number of reflections | 40046 | |
<I/σ(I)> | 18.9 | 8 |
Completeness [%] | 96.0 | 95.4 |
Redundancy | 4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.1 M sodium citrate, 0.2 M sodium/potassium tartrate, 1.4 M ammonium sulphate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |