2RCK
Crystal structure of juvenile hormone binding protein from Galleria mellonella hemolymph
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-12 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.00850, 1.00916, 0.8130 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 109.950, 109.950, 94.020 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 2.440 |
R-factor | 0.1945 |
Rwork | 0.191 |
R-free | 0.26400 |
Structure solution method | MAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.566 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.530 |
High resolution limit [Å] | 2.440 | 2.440 |
Rmerge | 0.062 | 0.538 |
Number of reflections | 24772 | |
<I/σ(I)> | 31.4 | 2.8 |
Completeness [%] | 99.5 | 97.3 |
Redundancy | 9.4 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 2.2M ammonium sulfate, 20mM sodium phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |