2RCK
Crystal structure of juvenile hormone binding protein from Galleria mellonella hemolymph
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-12 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.00850, 1.00916, 0.8130 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 109.950, 109.950, 94.020 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.440 |
| R-factor | 0.1945 |
| Rwork | 0.191 |
| R-free | 0.26400 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.566 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.530 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rmerge | 0.062 | 0.538 |
| Number of reflections | 24772 | |
| <I/σ(I)> | 31.4 | 2.8 |
| Completeness [%] | 99.5 | 97.3 |
| Redundancy | 9.4 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 2.2M ammonium sulfate, 20mM sodium phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
