2RCE
DFP modified DegS delta PDZ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | .97949 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.531, 132.745, 231.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.220 - 2.350 |
| R-factor | 0.19633 |
| Rwork | 0.194 |
| R-free | 0.23635 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.115 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.070 | 0.401 |
| Number of reflections | 90170 | |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 97.6 | 97.2 |
| Redundancy | 6.4 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 50 MM NA CACODYLATE PH 6.5, 100 MM NA CITRATE, 20% ISOPROPANOL, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
