2RC8
Crystal structure of the NR3A ligand binding core complex with D-serine at 1.45 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-23 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.790, 97.701, 60.050 |
| Unit cell angles | 90.00, 93.60, 90.00 |
Refinement procedure
| Resolution | 34.840 - 1.450 |
| R-factor | 0.15423 |
| Rwork | 0.153 |
| R-free | 0.17781 |
| Structure solution method | Difference Fourier |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.656 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.3.0038) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.050 | 0.292 |
| Number of reflections | 100689 | |
| <I/σ(I)> | 14.3 | 2.5 |
| Completeness [%] | 96.0 | 72.1 |
| Redundancy | 3.1 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 293 | 0.12 M NaSCN, 0.1M NaCitrate, 0.01 M EDTA, 11% PEG 3350, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
