2RC7
Crystal structure of the NR3A ligand binding core complex with glycine at 1.58 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-25 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.892, 97.568, 59.901 |
| Unit cell angles | 90.00, 93.55, 90.00 |
Refinement procedure
| Resolution | 29.890 - 1.580 |
| R-factor | 0.14905 |
| Rwork | 0.148 |
| R-free | 0.17714 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pb7 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.460 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0038) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.640 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.054 | 0.209 |
| Number of reflections | 74941 | |
| <I/σ(I)> | 11.7 | 5.6 |
| Completeness [%] | 95.9 | 75.3 |
| Redundancy | 3.8 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 293 | 0.1 M NaBr, 0.1 mM NaAcetate, 4% PEG 4000, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
