2RC6
Refined structure of FNR from Leptospira interrogans bound to NADP+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU ULTRAX 18 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-01-01 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.168, 112.251, 92.399 |
| Unit cell angles | 90.00, 93.19, 90.00 |
Refinement procedure
| Resolution | 33.532 - 2.700 |
| R-factor | 0.2367 |
| Rwork | 0.235 |
| R-free | 0.27590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.771 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 92.450 | 112.510 | 2.750 |
| High resolution limit [Å] | 2.610 | 8.250 | 2.610 |
| Rmerge | 0.110 | 0.045 | 0.734 |
| Number of reflections | 36768 | ||
| <I/σ(I)> | 6.3 | 11.6 | 1 |
| Completeness [%] | 87.8 | 99.9 | 67.4 |
| Redundancy | 3 | 3.8 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 291 | 27% PEG3350, 0.3M ammonium fluoride, 50mM Tris-HCL buffer, pH 7.0, vapor diffusion, temperature 291K |
