2RBM
Crystal structure of Staphylococcal nuclease variant Delta+PHS I72K at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2007-08-06 |
| Detector | BRUKER APEX II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.229, 60.603, 72.579 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.520 - 1.900 |
| R-factor | 0.17981 |
| Rwork | 0.173 |
| R-free | 0.23795 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1TQO.PDB where all waters removed all Bs=20.0 A^2 and I72A and E92A |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.950 |
| Data reduction software | SAINT |
| Data scaling software | SAINT (V7.34A) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.520 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 11779 | |
| <I/σ(I)> | 37.25 | 6.13 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 18.9 | 8.75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 33% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
