2RBG
Crystal structure of hypothetical protein(ST0493) from sulfolobus tokodaii
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-16 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97899, 0.9, 0.97931 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.444, 65.184, 49.604 |
Unit cell angles | 90.00, 98.19, 90.00 |
Refinement procedure
Resolution | 33.490 - 1.750 |
R-factor | 0.173 |
Rwork | 0.173 |
R-free | 0.19600 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.059 | 0.143 |
Number of reflections | 25105 | |
Completeness [%] | 99.6 | 96.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7.5 | 293 | 30% PEG 4K, 0.2M Ammonium Sulfate, pH 7.5, MICROBATCH, temperature 293K |