2RBB
Crystal structure of a glyoxalase/bleomycin resistance protein/dioxygenase family enzyme from Burkholderia phytofirmans PsJN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-09-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97930 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 83.230, 83.230, 99.590 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.930 - 1.820 |
R-factor | 0.239 |
Rwork | 0.239 |
R-free | 0.25900 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELX |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.890 |
High resolution limit [Å] | 1.820 | 1.820 |
Rmerge | 0.073 | |
Number of reflections | 32129 | |
<I/σ(I)> | 10.4 | 2.3 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 27.1 | 17.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 2M Sodium citrate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |