2RB9
Crystal structure of E.coli HypE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-19 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 254.574, 72.303, 112.607 |
| Unit cell angles | 90.00, 115.33, 90.00 |
Refinement procedure
| Resolution | 43.730 - 2.000 |
| R-factor | 0.177 |
| Rwork | 0.176 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2i6r |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.151 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 3.450 | 1.600 |
| Rmerge | 0.068 | 0.037 | 0.596 |
| Number of reflections | 196364 | ||
| <I/σ(I)> | 7.5 | ||
| Completeness [%] | 81.7 | 97.2 | 9.4 |
| Redundancy | 3.4 | 3.7 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 0.6M Na/K phosphate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
