2RAW
Crystal structure of the Borealin-Survivin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.700, 51.681, 46.289 |
| Unit cell angles | 90.00, 98.82, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.25354 |
| Rwork | 0.248 |
| R-free | 0.29829 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1F3H chain A |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.149 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 10387 | |
| <I/σ(I)> | 14.9 | 3 |
| Completeness [%] | 98.0 | 100 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 0.5 ul protein and 0.5 ul well solution (0.1 M Na HEPES, pH 7.5; 0.2 M CaCl2; 28% (w/v) PEG 400), VAPOR DIFFUSION, HANGING DROP, temperature 292K |
