2RAP
THE SMALL G PROTEIN RAP2A IN COMPLEX WITH GTP
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW21B |
| Synchrotron site | LURE |
| Beamline | DW21B |
| Temperature [K] | 278 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 |
| Unit cell lengths | 64.500, 64.500, 84.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.600 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.28100 |
| Structure solution method | DIFFERENCE FOURIER MAPS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.065 |
| Total number of observations | 46223 * |
| Number of reflections | 8828 |
| Completeness [%] | 98.5 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | 25% PEG 8000, 100MM LISO4, 100MM TRIS PH 8., pH 8.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 25 (%) | |
| 2 | 1 | reservoir | 100 (mM) | ||
| 3 | 1 | reservoir | Tris-HCl | 100 (mM) |
