2RAK
PI(3)P bound PX-BAR membrane remodeling unit of Sorting Nexin 9
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97955 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 64.059, 144.177, 118.245 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.890 - 3.000 |
R-factor | 0.2331 |
Rwork | 0.233 |
R-free | 0.29300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.400 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.890 | 3.100 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.057 | 0.372 |
Number of reflections | 12284 | |
<I/σ(I)> | 22.3 | 4.7 |
Completeness [%] | 99.4 | 99.8 |
Redundancy | 4.6 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.3M Ammonium tartrate dibasic, 0.1M BIS-TRIS propane, pH 7.0 , VAPOR DIFFUSION, HANGING DROP, temperature 293K |