2RAJ
SO4 bound PX-BAR membrane remodeling unit of Sorting Nexin 9
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97949 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 61.571, 147.477, 115.283 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.930 - 2.450 |
R-factor | 0.2411 |
Rwork | 0.241 |
R-free | 0.27600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 19.930 |
High resolution limit [Å] | 2.450 |
Rmerge | 0.038 |
Number of reflections | 20901 |
<I/σ(I)> | 22.9 |
Completeness [%] | 99.7 |
Redundancy | 9.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.3M Ammonium tartrate dibasic, 0.1M BIS-TRIS propane, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |