2RAG

Crystal structure of aminohydrolase from Caulobacter crescentus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-09-03
Detector	MAR CCD 165 mm
Wavelength(s)	0.97915
Spacegroup name	P 1 21 1
Unit cell lengths	79.672, 129.238, 99.226
Unit cell angles	90.00, 104.76, 90.00

Refinement procedure

Resolution	24.980 - 2.000
R-factor	0.238
Rwork	0.238
R-free	0.25000
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.000
Number of reflections	120726
Completeness [%]	92.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	25% PEG 3350, 0.1M Bis-Tris, 0.2M Lithium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K