2RAF
Crystal structure of putative dinucleotide-binding oxidoreductase (NP_786167.1) from Lactobacillus plantarum at 1.60 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-07-25 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91837, 0.97886, 0.97917 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 53.330, 152.570, 140.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.540 - 1.600 |
R-factor | 0.153 |
Rwork | 0.151 |
R-free | 0.18600 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.751 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SOLVE |
Refinement software | REFMAC (5.3.0040) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.540 | 29.540 | 1.660 |
High resolution limit [Å] | 1.600 | 3.440 | 1.600 |
Rmerge | 0.053 | 0.020 | 0.368 |
Number of reflections | 75349 | 14311 | 14986 |
<I/σ(I)> | 10.8 | 32.7 | 2.2 |
Completeness [%] | 99.2 | 97.4 | 98.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | NANODROP, 20.0% PEG 3350, 0.2M Ammonium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 293K |