2R9G
Crystal structure of the C-terminal fragment of AAA ATPase from Enterococcus faecium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-31 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 |
| Unit cell lengths | 82.778, 103.390, 103.374 |
| Unit cell angles | 90.01, 88.69, 86.05 |
Refinement procedure
| Resolution | 20.000 - 2.090 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qw6 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.151 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.126 | 0.760 |
| Number of reflections | 202788 | |
| <I/σ(I)> | 2.8 | 2.1 |
| Completeness [%] | 87.3 | 44.4 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 294 | 100mM HEPES pH 7.5, 25% PEG 3350, 200mM Ammonium acetate, temperature 294K |
