2R8B
The crystal structure of the protein Atu2452 of unknown function from Agrobacterium tumefaciens str. C58
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97924 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.904, 58.727, 165.155 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.840 - 2.560 |
| R-factor | 0.19735 |
| Rwork | 0.194 |
| R-free | 0.25250 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.908 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.500 | 2.630 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.092 | 0.487 |
| Number of reflections | 18017 | |
| <I/σ(I)> | 31.4 | 2.85 |
| Completeness [%] | 93.7 | 58.1 |
| Redundancy | 6.4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 289 | 0.1M Na Citrate, 0.2M K/Na Tartrate, 2.0M Ammonium sulfate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
