2R80
Pigeon Hemoglobin (OXY form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-13 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.086, 81.543, 79.214 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.810 - 1.440 |
| R-factor | 0.19868 |
| Rwork | 0.197 |
| R-free | 0.23464 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1faw |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.232 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.530 |
| High resolution limit [Å] | 1.440 | 1.440 |
| Number of reflections | 87504 | |
| <I/σ(I)> | 15.5 | 4.8 |
| Completeness [%] | 93.7 | 95.2 |
| Redundancy | 7.3 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PEG 3350, TRIS-HCL BUFFER, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
