2R7Y
Selenium Derivatized RNA/DNA Hybrid in complex with RNase H CATALYTIC DOMAIN MUTANT D132N
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9797,0.9800,0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 80.660, 37.819, 61.937 |
| Unit cell angles | 90.00, 96.60, 90.00 |
Refinement procedure
| Resolution | 23.450 - 1.800 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| R-free | 0.23100 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.060 | 0.278 |
| Number of reflections | 17227 | |
| <I/σ(I)> | 15.3 | 10.5 |
| Completeness [%] | 98.6 | 98.6 |
| Redundancy | 7.3 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1 M MES, 12% PEG 20000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MES | ||
| 2 | 1 | 1 | PEG 20000 | ||
| 3 | 1 | 2 | MES | ||
| 4 | 1 | 2 | PEG 20000 |
