2R7D
Crystal structure of ribonuclease II family protein from Deinococcus radiodurans, triclinic crystal form. NorthEast Structural Genomics target DrR63
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-07-16 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.97900 |
| Spacegroup name | P 1 |
| Unit cell lengths | 65.762, 92.205, 92.345 |
| Unit cell angles | 60.08, 89.57, 71.11 |
Refinement procedure
| Resolution | 36.680 - 1.800 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.24300 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.044 | 0.307 |
| Number of reflections | 308057 | |
| <I/σ(I)> | 15.8 | 14 |
| Completeness [%] | 96.0 | 88 |
| Redundancy | 2 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | MgCl2, Tris-HCl, 30% PEG 8000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
