2R77
Crystal structure of phosphatidylethanolamine-binding protein, pfl0955c, from Plasmodium falciparum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ DW |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-08-31 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54180 |
| Spacegroup name | P 41 |
| Unit cell lengths | 54.229, 54.229, 69.927 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.610 - 1.650 |
| R-factor | 0.187 |
| Rwork | 0.185 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gzq |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.382 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.055 | 0.435 |
| Number of reflections | 22535 | |
| <I/σ(I)> | 19.4 | 2.83 |
| Completeness [%] | 92.2 | 56.5 |
| Redundancy | 6.8 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 30% PEG 3350, 0.2 M Sodium chloride, 0.1 M Sodium cacodylate pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
