2R72
Crystal structure of infectious bursal disease virus VP1 polymerase, incubated with Mg2+ ion.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-02 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.933 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 122.838, 122.838, 354.255 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 3.150 |
R-factor | 0.211 |
Rwork | 0.209 |
R-free | 0.23500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2pus |
RMSD bond length | 0.006 |
RMSD bond angle | 0.962 |
Data scaling software | SCALA |
Phasing software | REFMAC |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 36.664 | 36.660 | 3.320 |
High resolution limit [Å] | 3.150 | 9.960 | 3.150 |
Rmerge | 0.121 | 0.055 | 0.356 |
Total number of observations | 4106 | 16753 | |
Number of reflections | 27660 | ||
<I/σ(I)> | 5.1 | 10.3 | 1.9 |
Completeness [%] | 98.4 | 93.6 | 98.6 |
Redundancy | 6.5 | 4.3 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 10% PEG 3350, 0.4M LiNO3, 0.1M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |