2R71
Crystal structure of the complex of bovine C-lobe with inositol at 2.1A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-08-25 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.483, 50.314, 65.974 |
| Unit cell angles | 90.00, 107.84, 90.00 |
Refinement procedure
| Resolution | 62.750 - 2.070 |
| R-factor | 0.18545 |
| Rwork | 0.183 |
| R-free | 0.22933 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b65 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.313 |
| Data reduction software | DENZO |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.750 | 2.140 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Number of reflections | 22672 | |
| <I/σ(I)> | 5.4 | 14.9 |
| Completeness [%] | 98.0 | 93.7 |
| Redundancy | 4.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1M MES, 25% POLYETHYLENE GLYCOL MONOMETHYL ETHER 550, 0.01M ZINC SULPHATE, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
