2R70
Crystal structure of infectious bursal disease virus VP1 polymerase, cocrystallized with an oligopeptide mimicking the VP3 C-terminus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.975 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 123.801, 123.801, 361.843 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.700 |
| R-factor | 0.223 |
| Rwork | 0.221 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.257 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 107.215 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.100 | 0.434 |
| Total number of observations | 78074 | |
| Number of reflections | 43251 | |
| <I/σ(I)> | 4.9 | 1.7 |
| Completeness [%] | 95.0 | 96.3 |
| Redundancy | 12.2 | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 293 | 5% PEG 3350, 0.4M LiNO3, 0.1M Tris, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
