2R6Z
Crystal structure of the SAM-dependent methyltransferase NGO1261 from Neisseria gonorrhoeae, Northeast Structural Genomics Consortium Target NgR48
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-08-29 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97893 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.233, 43.186, 103.570 |
Unit cell angles | 90.00, 97.54, 90.00 |
Refinement procedure
Resolution | 26.820 - 1.800 |
R-factor | 0.215 |
Rwork | 0.215 |
R-free | 0.23100 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.820 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.040 | 0.365 |
Number of reflections | 91274 | |
<I/σ(I)> | 28.9 | 2.96 |
Completeness [%] | 98.4 | 96.6 |
Redundancy | 4.1 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH UNDER OIL | 6.5 | 277 | Protein solution: 10 mM Tris-HCl pH 7.5, 100 mM NaCl, 5 mM DTT. Reservoir solution: 100 mM Cacodylic acid pH 6.5, 18% PEG 3350, MICROBATCH UNDER OIL, temperature 277K |