2R6V

Crystal structure of FMN-binding protein (NP_142786.1) from Pyrococcus horikoshii at 1.35 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-07-20
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837, 0.97939, 0.97910
Spacegroup name	P 61 2 2
Unit cell lengths	46.200, 46.200, 267.590
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.588 - 1.250
R-factor	0.128
Rwork	0.127
R-free	0.15400
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.549
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.588	44.600	1.290
High resolution limit [Å]	1.250	2.690	1.250
Rmerge	0.068	0.058	0.318
Number of reflections	41184	5312	1197
<I/σ(I)>	23.93	44	3.2
Completeness [%]	84.5	99.4	28.2
Redundancy	18.3	27	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.6	277	NANODROP, 0.2M Ammonium formate, 20.0% PEG 3350, No Buffer pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K