2R6U
Crystal structure of gene product RHA04853 from Rhodococcus sp. RHA1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97940 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.411, 89.048, 58.913 |
| Unit cell angles | 90.00, 94.09, 90.00 |
Refinement procedure
| Resolution | 58.720 - 1.500 |
| R-factor | 0.17786 |
| Rwork | 0.176 |
| R-free | 0.21076 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.550 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.720 | 1.538 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.072 | 0.343 |
| Number of reflections | 79299 | |
| <I/σ(I)> | 32.3 | 2.85 |
| Completeness [%] | 98.7 | 88.54 |
| Redundancy | 9.5 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 0.1M Na(OAC), 0.2M Ammonium acetate, 30% PEG 4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
