2R6R
Aquifex aeolicus FtsZ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.644, 73.944, 43.813 |
| Unit cell angles | 90.00, 95.08, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.18104 |
| Rwork | 0.179 |
| R-free | 0.22691 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fsz |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.418 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.700 |
| Rmerge | 0.046 |
| Number of reflections | 30532 |
| <I/σ(I)> | 16.4 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292 | 15 g/L FtsZ, 2 mM MgCl2, 1 mM GDP, 0.1 M MOPS-NaOH, 0.2 M NaCl, 28% (v/v) PEG400, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
