2R6F
Crystal Structure of Bacillus stearothermophilus UvrA
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Wavelength(s) | 0.97925, 0.97941, 0.96403 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 102.713, 94.720, 130.481 |
Unit cell angles | 90.00, 108.80, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.200 |
R-factor | 0.257 |
Rwork | 0.253 |
R-free | 0.29200 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.247 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.310 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.079 | 0.305 |
Number of reflections | 38657 | |
<I/σ(I)> | 13.4 | |
Completeness [%] | 98.3 | 85.9 |
Redundancy | 6.9 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 13%PEG2000MME, 50 mM Na-HEPES pH 7.0, 500 mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 13%PEG2000MME, 50 mM Na-HEPES pH 7.0, 500 mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 13%PEG2000MME, 50 mM Na-HEPES pH 7.0, 500 mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 295K |