2R66
Complex Structure of Sucrose Phosphate Synthase (SPS)-F6P of Halothermothrix orenii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-06-13 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 154.225, 48.499, 75.051 |
Unit cell angles | 90.00, 100.92, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.800 |
Rwork | 0.210 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2r60 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.062 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 6.030 | 2.800 |
Rmerge | 0.075 | 0.033 | 0.282 |
Number of reflections | 13734 | ||
<I/σ(I)> | 14.6 | ||
Completeness [%] | 99.8 | 98.5 | 100 |
Redundancy | 3.7 | 3.5 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 20% PEG 4000, 0.6M NaCl, 0.1M Na MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |