2R62
Crystal structure of Helicobacter pylori ATP dependent protease, FtsH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2006-05-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 64 |
| Unit cell lengths | 141.217, 141.217, 54.659 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.300 |
| R-factor | 0.28283 |
| Rwork | 0.280 |
| R-free | 0.33596 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ixz |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.799 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.380 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.065 | 0.391 |
| Number of reflections | 8963 | |
| <I/σ(I)> | 41.1 | 3.7 |
| Completeness [%] | 98.0 | 99.3 |
| Redundancy | 3.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.1M Tris-HCl pH 8.0, 17%(w/v) PEG 4000, 0.2M Li2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
